Anti-FFAR3 antibody (ab103718)

Overview

  • Product nameAnti-FFAR3 antibody
    See all FFAR3 primary antibodies
  • Description
    Rabbit polyclonal to FFAR3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 301-331 of Human FFAR3.

  • Positive control
    • Jurkat cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell line: T24/83.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab103718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate > acetate > formate.
  • Tissue specificityHighest level in adipose tissue, and lower expression across all tissues tested.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FFA3R antibody
    • Ffar3 antibody
    • FFAR3_HUMAN antibody
    • Free fatty acid receptor 3 antibody
    • G protein coupled receptor 41 antibody
    • G-protein coupled receptor 41 antibody
    • gpcr41 antibody
    • GPR41 antibody
    • gpr42 antibody
    see all

Anti-FFAR3 antibody images

  • ICC/IF image of ab103718 stained T24/83 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103718 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FFAR3 antibody (ab103718) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 39 kDa

References for Anti-FFAR3 antibody (ab103718)

ab103718 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Sample Rabbit Cell (GPR41 transfacted CHO-K1 cell)
Specification GPR41 transfacted CHO-K1 cell
Permeabilization Yes - 0.1% tween 20
Fixative Formaldehyde
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Verified customer

Submitted Apr 01 2015

Gracias por contactarnos de nuevo.
El péptido usado para generar el anticuerpo es el producto ab128002, podéis echarle un vistazo a su datasheet:
http://www.abcam.com/ffar3-peptide-ab128002.html
Si necesitas cualquier otra información, p...

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Thank you for contacting us.

I apologize for the time that it has taken to get this onto our catalog. We do now offer the blocking peptide ab103718 anti-FFAR3 (C-term). This product number is ab128002 and can be located at the following li...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"