Overview

  • Product nameAnti-FGD2 antibody
    See all FGD2 primary antibodies
  • Description
    Rabbit polyclonal to FGD2
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: VTVFENSRTP EAAPRGQRLE DVHHRPECRP PESPGPREKT NVGEAVGSEP RTVSRRYLNS LKNKLSSEAW RKSCQPVTLS GSGTQEPEKK IVQEL, corresponding to amino acids 16-110 of Human FGD2

  • Positive control
    • Human kidney tissue, Over-expression HEK293T Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActivates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 FYVE-type zinc finger.
    Contains 2 PH domains.
  • DomainThe FYVE-type zinc-finger is necessary for early endosome localization. Recruitement to endosomal membranes via this domain requires the presence of phosphatidylinositol 3-phosphate or other phosphatidylinositides.
    The PH domain is necessary for localization to the ruffle membrane. Recruitement to ruffle membrane occurs through binding of phosphoinositides by the PH domain. This domain also contributes to the lipid-binding properties of the protein.
    The DH domain is necessary for its ability to activate JNK1 via CDC42.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm. Nucleus. Early endosome. Early endosome membrane. Cell projection > ruffle membrane. Recruitement to the endosome and ruffle membrane requires the presence of phosphoinositides.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGD1 family member 2 antibody
    • Fgd2 antibody
    • FGD2_HUMAN antibody
    • FLJ00276 protein antibody
    • FYVE antibody
    • FYVE RhoGEF and PH domain containing 2 antibody
    • RhoGEF and PH domain-containing protein 2 antibody
    • ZFYVE4 antibody
    • Zinc finger FYVE domain-containing protein 4 antibody
    see all

Anti-FGD2 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122040 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122040, at 1/100, staining FGD2 in paraffin embedded Human kidney tissue by Immunohistochemistry.

References for Anti-FGD2 antibody (ab122040)

ab122040 has not yet been referenced specifically in any publications.

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