Overview

  • Product name
    Anti-FGD4 antibody
  • Description
    Rabbit polyclonal to FGD4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 49-297 of Human FGD4 (NP_640334).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; SG xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab97785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8.
  • Tissue specificity
    Expressed in different tissues, including brain, cerebellum, peripheral nerve, skeletal muscle, heart, uterus, placenta and testis.
  • Involvement in disease
    Charcot-Marie-Tooth disease 4H (CMT4H) [MIM:609311]: A recessive demyelinating form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Demyelinating neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 FYVE-type zinc finger.
    Contains 2 PH domains.
  • Domain
    The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > filopodium. Concentrated in filopodia and poorly detected at lamellipodia. Binds along the sides of actin fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9030023J02Rik antibody
    • 9330209B17Rik antibody
    • Actin filament binding protein frabin antibody
    • Actin filament-binding protein frabin antibody
    • CMT4H antibody
    • DKFZp313E1818 antibody
    • DKFZp434K1572 antibody
    • FGD1 related F actin binding protein antibody
    • FGD1-related F-actin-binding protein antibody
    • Fgd4 antibody
    • FGD4_HUMAN antibody
    • FLJ34370 antibody
    • FLJ42663 antibody
    • FRABP antibody
    • FYVE antibody
    • FYVE, RhoGEF and PH domain containing 4 antibody
    • MGC57222 antibody
    • RhoGEF and PH domain-containing protein 4 antibody
    • ZFYVE6 antibody
    • Zinc finger FYVE domain containing protein 6 antibody
    • Zinc finger FYVE domain-containing protein 6 antibody
    see all

Images

  • Anti-FGD4 antibody (ab97785) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 87 kDa
  • ab97785 at 1/200 dilution staining FGD4 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
  • ab97785 at 1/100 dilution staining FGD4 in SG xenograft by Immunohistochemistry, Paraffin-embedded tissue.

References

This product has been referenced in:
  • Ottman R  et al. MicroRNA expressions associated with progression of prostate cancer cells to antiandrogen therapy resistance. Mol Cancer 13:1 (2014). WB ; Human . Read more (PubMed: 24387052) »

See 1 Publication for this product

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