Anti-FGF 23 antibody (ab123502)
Key features and details
- Goat polyclonal to FGF 23
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-FGF 23 antibody
See all FGF 23 primary antibodies -
Description
Goat polyclonal to FGF 23 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide with sequence C-
ENGYDVYLSQKHH
, corresponding to internal sequence amino acids 121-133 of Mouse FGF 23 (NP_073148.1) -
Positive control
- Mouse Brain and testes lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 27, 38 kDa (predicted molecular weight: 28 kDa).
A 1 hour primary incubation is recommended for this product. When using mouse brain lysate: |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 27, 38 kDa (predicted molecular weight: 28 kDa). A 1 hour primary incubation is recommended for this product. When using mouse brain lysate: |
Target
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Function
Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. -
Tissue specificity
Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts). -
Involvement in disease
Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Post-translational
modificationsFollowing secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. -
Cellular localization
Secreted. Secretion is dependent on O-glycosylation. - Information by UniProt
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Database links
- Entrez Gene: 64654 Mouse
- Entrez Gene: 170583 Rat
- SwissProt: Q9EPC2 Mouse
- SwissProt: Q8VI82 Rat
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Alternative names
- ADHR antibody
- FGF-23 antibody
- Fgf23 antibody
see all
Images
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Anti-FGF 23 antibody (ab123502) at 0.5 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. -
All lanes :
Lane 1 : Mouse Testes Lysate 1 in RIPA buffer
Lane 2 : Mouse Testes Lysate 2 in RIPA buffer
Lane 3 : Mouse Testes Lysate in RIPA buffer with blocking peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Datasheets and documents
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Datasheet download
References (0)
ab123502 has not yet been referenced specifically in any publications.