Overview

  • Product nameFGF basic Mouse ELISA KitSee all FGF basic kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    18.8 pg/ml - 600 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 119.7 105% - 129%
    Serum 84.63 78% - 100%
    Plasma 83.26 76% - 99%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s FGF basic Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse FGF basic in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceFGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
  • Alternative names
    • Basic fibroblast growth factor
    • BFGF
    • FGF 2
    • FGF B
    • FGF2
    • FGF2 basic
    • FGFB
    • Fibroblast growth factor 2 (basic)
    • HBGF 2
    • HBGF2
    • HBGH 2
    • Heparin binding growth factor 2
    • Heparin binding growth factor 2 precursor
    • OTTMUSP00000046776
    • Prostatropin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

FGF basic Mouse ELISA Kit images

  • Mouse FGF standard curve: mean of duplicates (+/- SD), performed in Diluent A

  • Mouse FGF measured in biological fluids showing quantity (pg) per mL of tested sample.
  • Representative Standard Curve using ab100670.

  • Representative Standard Curve using ab100670.

Protocols

References for FGF basic Mouse ELISA Kit (ab100670)

ab100670 has not yet been referenced specifically in any publications.

Product Wall

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I assume you are looking for a lysis buffer because you wish to test cell or tissue lysates samples with the kit? If so, please note that the ab100670 kit has not been validated with lysate samples, only serum, plasm...

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Although this kit was neither designed nor tested with cell or tissue lysate samples, this kit as well as our other sandwich ELISA kits are very accommodating to many different sample types so lysates samples shou...

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Thank you for contacting us. We do not currently sell any ELISA kits that are specifically designed for use on rat samples. After checking with the lab, our mouse and human ELISA kits for these targets will be unlikely to cross-react with rat, but have...

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Unfortunately, epitope mapping of the two antibody pairs has not been performed, so we do not know the exact amino acid sequence recognised and if these regions are specific for human and mouse, respect...

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Thank you for your phone call today. I have heard back from my contact at the laboratory, and I was told that the ELISA plates come pre-blocked so a blocking step is not necessary.  I hope this information is helpful, but please let me know if y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"