Anti-FGF1 alpha antibody (ab169748)
Key features and details
- Mouse polyclonal to FGF1 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF1 alpha antibody -
Description
Mouse polyclonal to FGF1 alpha -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Chicken, Hamster, Cow, Pig, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human FGF1 alpha aa 1-155.
Database link: NP_000791.1 -
Positive control
- FGF1 alpha transfected 293T cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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Database links
- Entrez Gene: 2246 Human
- Entrez Gene: 14164 Mouse
- Entrez Gene: 25317 Rat
- Omim: 131220 Human
- SwissProt: P19596 Chicken
- SwissProt: P03968 Cow
- SwissProt: P34004 Hamster
- SwissProt: P05230 Human
see all -
Alternative names
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta-endothelial cell growth factor antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling FGF1 alpha with ab169748 at 10µg/ml.
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All lanes : Anti-FGF1 alpha antibody (ab169748) at 1 µg/ml
Lane 1 : FGF1 alpha transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 17 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab169748 has been referenced in 3 publications.
- Li R et al. Acidic fibroblast growth factor attenuates type 2 diabetes-induced demyelination via suppressing oxidative stress damage. Cell Death Dis 12:107 (2021). PubMed: 33479232
- Ichikawa K et al. Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy. Sci Rep 10:2939 (2020). PubMed: 32076044
- Han J et al. Acid fibroblast growth factor facilitates the progression of atherosclerotic plaques regardless of alterations in serum lipid expression levels in HFD-fed ApoE-/- mice. Mol Med Rep 18:1025-1030 (2018). PubMed: 29845277