Overview

  • Product nameAnti-FGF1 antibody [2E12]
    See all FGF1 primary antibodies
  • Description
    Mouse monoclonal [2E12] to FGF1
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human FGF1 protein.

  • Positive control
    • Human placenta tissue. HeLa cells. Tranfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab117640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

Anti-FGF1 antibody [2E12] images

  • Overlay histogram showing HEK293 cells stained with ab117640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117640, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Human Placenta, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117640 at 5µg/ml.
  • Immunofluorescence analysis of FGF1 expression on HeLa cells with ab117640 at 10µg/ml.
  • All lanes : Anti-FGF1 antibody [2E12] (ab117640)

    Lane 1 : Transfected 293T cell line
    Lane 2 : Non-Transfected 293T cell line


    Predicted band size : 17 kDa
  • The detection limit for ab117640 is approximately 10ng/ml as a capture antibody.

References for Anti-FGF1 antibody [2E12] (ab117640)

ab117640 has not yet been referenced specifically in any publications.

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