• Product nameAnti-FGF1 antibody [2E12]
    See all FGF1 primary antibodies
  • Description
    Mouse monoclonal [2E12] to FGF1
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human FGF1 protein.

  • Positive control
    • Human placenta tissue. HeLa cells. Tranfected 293T cell lysate.



Our Abpromise guarantee covers the use of ab117640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

Anti-FGF1 antibody [2E12] images

  • Overlay histogram showing HEK293 cells stained with ab117640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117640, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Human Placenta, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117640 at 5µg/ml.
  • Immunofluorescence analysis of FGF1 expression on HeLa cells with ab117640 at 10µg/ml.
  • All lanes : Anti-FGF1 antibody [2E12] (ab117640)

    Lane 1 : Transfected 293T cell line
    Lane 2 : Non-Transfected 293T cell line

    Predicted band size : 17 kDa
  • The detection limit for ab117640 is approximately 10ng/ml as a capture antibody.

References for Anti-FGF1 antibody [2E12] (ab117640)

ab117640 has not yet been referenced specifically in any publications.

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