Our Abpromise guarantee covers the use of ab9588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hFGF1 (30.0 ng/ml), a concentration of 0.46 - 0.9 µg/ml of this antibody is required.
IHC-Fr 1/200.
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

Anti-FGF1 antibody images

  • ICC/IF image of ab9588 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab9588 staining FGF1 in rat dorsal root ganglia tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and permeabilized using 0.25% Triton X-100. Samples were then blocked 0.25% BSA for 1 hour at 25°C, then incubated with ab9588 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to rhodamine, 1/500 dilution.

    See Abreview

References for Anti-FGF1 antibody (ab9588)

This product has been referenced in:
  • Li M  et al. FGF1 Mediates Overnutrition-Induced Compensatory ß-Cell Differentiation. Diabetes 65:96-109 (2016). IF ; Zebrafish . Read more (PubMed: 26420862) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (DRG)
Specification DRG
Fixative Paraformaldehyde
Permeabilization Yes - 0,25% Trt-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C

Mrs. Duygu Daglikoca

Verified customer

Submitted Jun 06 2011

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Loading amount 20 µg
Specification Heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 02 2008

This antibody has never been tested in IHC on formalin fixed paraffin embedded sections, therfore we can't guarantee results.