Anti-FGF12 antibody (ab58157)

Overview

  • Product name
  • Description
    Mouse monoclonal to FGF12
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-181 of Human FGF12 (isoform 2).

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab58157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probably involved in nervous system development and function.
    • Tissue specificity
      Brain, eye and testis; highly expressed in embryonic retina, olfactory epithelium, olfactory bulb, and in a segmental pattern of the body wall; in adult olfactory bulb, less in cerebellum, deep cerebellar nuclei, cortex and multiple midbrain structures.
    • Sequence similarities
      Belongs to the heparin-binding growth factors family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EIEE47 antibody
      • FGF-12 antibody
      • Fgf12 antibody
      • FGF12_HUMAN antibody
      • FGF12B antibody
      • FHF-1 antibody
      • FHF1 antibody
      • Fibroblast growth factor 12 antibody
      • Fibroblast growth factor 12B antibody
      • Fibroblast growth factor FGF 12b antibody
      • Fibroblast growth factor homologous factor 1 antibody
      • Myocyte activating factor antibody
      • Myocyte-activating factor antibody
      see all

    Images

    • FGF12 antibody (ab58157) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.
    • Western blot against tagged recombinant protein immunogen using ab58157 FGF12 antibody at 1ug/ml. Predicted band size of immunogen is 46 kDa

    References

    ab58157 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Mr. Jeff Guo

    Verified customer

    Submitted Aug 22 2017

    Thank you very much for your call today and for letting us know about the trouble with these antibodies.

    I'm sending a free of charge vial of ab135603 to replace ab58157. We expect this to arrive around Wednesday of next week on the order ***...

    Read More

    Thank you for your reply and for your patience.
    I have just been informed that, as requested, the full length FGF12 protein will be added to our catalog. The price is €625.00 for 10µg. This product should be available for order next week under the...

    Read More

    Thank you for your reply.

    I have checked our records and the email address used on the 23rd is yours, **@***.**. It happens sometimes that our emails are directed to the junk email folder.
    About the price of the protein, I will be able t...

    Read More

    Thank you for your email.
    Please find below a copy of the email I sent to you on Wednesday 23rd of May:
    "we have been informed that unfortunately there is no blocking peptide available for ab58157, clone 1D9.
    The recombinant protein used...

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    Regarding your enquiry, we have been informed that unfortunately there is no blocking peptide available for ab58157, clone 1D9.
    The recombinant protein used to generate the antibody is FGF12 (Human) Recombinant Protein (aa 1-181) with a tag at the...

    Read More

    Thank you for contacting us.
    a) Looking at our archives, the anti-FGF12 ab58157 has never been tested with Mouse. I think this product was predicted to react with Mouse since the homology between the immunogen (aa 1-182) and the Mouse protein is 1...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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