The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Detection limit for recombinant Human protein: 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to FGF17 (ab9990). Detection limit for recombinant Human protein: 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Can be used as detection antibody when paired with ab9990.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa. Detection limit for recombinant Human protein: 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.
FunctionPlays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
Tissue specificityPreferentially expressed in the embryonic brain.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Developmental stageDetected in embryos at E14.5, but not at E10.5 and E19.5. Preferentially expressed in the neuroepithelia of the isthmus and septum of the embryonic brain at E14.5.