Overview

  • Product nameAnti-FGF18 antibody - N-terminal
    See all FGF18 primary antibodies
  • Description
    Rabbit polyclonal to FGF18 - N-terminal
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of Human FGF18, conjugated to KLH

  • Positive control
    • NCI-H460 cell line lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab86571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 µg/ml.
WB 1/1000. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionPlays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGF 18 antibody
    • FGF-18 antibody
    • Fgf18 antibody
    • FGF18_HUMAN antibody
    • Fibroblast growth factor 18 antibody
    • zFGF5 antibody
    see all

Anti-FGF18 antibody - N-terminal images

  • ICC/IF image of ab86571 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86571 at 0.5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-FGF18 antibody - N-terminal (ab86571) + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 24 kDa

References for Anti-FGF18 antibody - N-terminal (ab86571)

This product has been referenced in:
  • Hamidouche Z  et al. Autocrine fibroblast growth factor 18 mediates dexamethasone-induced osteogenic differentiation of murine mesenchymal stem cells. J Cell Physiol 224:509-15 (2010). Read more (PubMed: 20432451) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in Abcam FGF18 antibodies.

The immunogen of http://www.abcam.com/ab63037 has 82% homology with the Chicken protein. However, immunogens of both http://www.abcam.com/ab86571 and http://www.abcam.com/ab13390...

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Thank you for your enquiry.

I am can confirm that ab86571 FGF18 antibody is sourced externally, and regrettably the exact immunogen sequence is proprietary (commercially sensitive) on this occasion.

However, we can let you know th...

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Thank you for your enquiry. Unfortunately, we have not tested ab86571 with mouse FGF18 yet. But, sequential homology between the immunogen sequence of ab86571 and mouse FGF18 (SwissProt O89101) is 100%. So, there is good possibility that ab86571 can wo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"