• Product name
  • Description
    Rabbit polyclonal to FGF2
  • Specificity
    Does not cross react with recombinant mouse and human IL6, recombinant human IL1 alpha and IL1 beta, recombinant human TNF alpha, recombinant human IL3, and recombinant human FGF acidic.
  • Tested applications
    Suitable for: Dot blot, ELISA, Neutralising, WB, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).



Our Abpromise guarantee covers the use of ab10420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/10000.
ELISA Use at an assay dependent dilution.
Neutralising Use a concentration of 5 µg/ml. 5 µg/ml neutralizes 50% of the biological activity of 1 unit of bFGF using the mouse fibroblast cell line 3T3.
WB Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
RIA Use at an assay dependent dilution.


  • Function
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificity
    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localization
    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all


ab10420 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.This antibody is usually diluted in tissue culture medium containing 10% serum, or in buffered saline containing 1% BSA depending upon your application. We don't have any particular information on the stability of this antib...

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Thank you for your phone call. I have found out that the tube you received was mislabeled as monoclonal instead of polyclonal. I have confirmed that ab10420 is indeed a polyclonal antibody as stated on our online datasheet. I apologize for this erro...

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