Overview

  • Product name
  • Description
    Rabbit polyclonal to FGF2
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    GFFLRIHPDGRVDGVREKSDPHIK

    , corresponding to amino acids 40-63 of Human FGF basic.

  • Positive control
    • IHC-P: Human fetal liver or kidney tissue; Human hepatitis, cirrhosis and HCC liver and pancreas tissues;.

Properties

Applications

Our Abpromise guarantee covers the use of ab16828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 22302773
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 18 kDa.

Target

  • Function
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificity
    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localization
    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Anti-FGF2 antibody images

  • Formaldehyde-fixed, paraffin-embedded human pancreas tissue stained for FGF using ab16828 at 1/50 dilution in immunohistochemical analysis.

    See Abreview

  • Paraffin-embedded human liver tissue stained for FGF using ab16828. A case of chronic hepatitis showing a few positive hepatocytes in (a) periportal areas (×200), (b) the pericentral zone (×400) and (c) intermediate hepatocytes (arrow) (×200). In HCC, (d) positivity was also detected in HCC tumor cells (×200). 

    PMID: 22839096

  • ab16828 at 5ug/ml staining human B lineage lymphoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 1 hour. Bound antibody was then detected using a biotinylated horse anti-rabbit and mouse IgG antibody.

    This image is courtesy of an Abreview submitted by Francisca Wu on 15 March 2006.

    See Abreview

References for Anti-FGF2 antibody (ab16828)

This product has been referenced in:
  • Han W  et al. The osteogenic potential of human bone callus. Sci Rep 6:36330 (2016). IHC ; Human . Read more (PubMed: 27796345) »
  • Zhang X  et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »

See all 8 Publications for this product

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Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha s...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
10% NBF
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.1
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: .25% · Temperature: 25°C
Username

Ms. Elizabeth Chlipala

Verified customer

Submitted Oct 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (tendon)
Specification
tendon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrat puffer ph 6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Feb 06 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (b-lineage lymphoma)
Specification
b-lineage lymphoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Francisca Wu

Verified customer

Submitted Mar 15 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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