Overview

  • Product nameAnti-FGF2 antibody
    See all FGF2 primary antibodies
  • Description
    Sheep polyclonal to FGF2
  • SpecificityCross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not been tested. Specificity was demonstrated by immunohistochemistry.
  • Tested applicationsSuitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human basic Fibroblast Growth Factor.

Properties

Applications

Our Abpromise guarantee covers the use of ab6209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
  • Application notesImmunohistochemistry: 1/2,000
    Western Blot: Not tested.
    Immunoblot: Not tested.
    1 site ELISA: Not tested.
    Biological Inhibition: Not tested.
  • Target

    • FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
    • Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
    • Sequence similaritiesBelongs to the heparin-binding growth factors family.
    • Post-translational
      modifications
      Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
      Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
    • Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    References for Anti-FGF2 antibody (ab6209)

    ab6209 has not yet been referenced specifically in any publications.

    Product Wall

    This antibody basic FGF (sheep) works well in tissues that have been fixed in Zamboni's fixative. This is 15% saturated picric acid in 4% paraformaldehyde in phosphate buffer. Antigen retrieval is not required.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"