Overview

  • Product nameAnti-FGF2 antibody
    See all FGF2 primary antibodies
  • Description
    Rabbit polyclonal to FGF2
  • SpecificityThis antibody recognises FGF basic (FGF-2)
  • Tested applicationsSuitable for: Dot blot, ICC, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: all Mammals
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-23 of Rat FGF basic.

  • Positive control
    • ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab8880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21187124
ICC/IF 1/200.

Target

  • FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Anti-FGF2 antibody images

  • ICC/IF image of ab8880 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8880, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of mouse brain tissue (Frozen sections) labelling FGF2 with ab8880 at a dilution of 1/500. Tissue sections were fixed with Paraformaldehyde and permeabilized with 0.3 % Troton X-100. The secondary antibody used was Alexa Fluor® 594 conjugated goat polyclonal secondary at 1/400. 

    See Abreview

  • ab8880 staining FGF basic in Mouse olfactory epithelium tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 1% blocking reagent from a tyramide signal amplification kit for 30 minutes at 23°C. The primary antibody was diluted 1/200 in the blocking reagent and incubated with the sample for 2 hours at 23°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/100.
    After secondary antibody incubation, the signal was amplified using a tyramide signal amplication kit with Alexa Fluor® 488 tyramide.

    See Abreview

References for Anti-FGF2 antibody (ab8880)

This product has been referenced in:
  • Park IS  et al. Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation. PLoS One 10:e0122776 (2015). WB, IHC-Fr ; Human . Read more (PubMed: 26065900) »
  • Rotschafer JH  et al. Modulation of neural stem/progenitor cell proliferation during experimental Herpes Simplex encephalitis is mediated by differential FGF-2 expression in the adult brain. Neurobiol Dis 58:144-55 (2013). IHC-FoFr ; Mouse . Read more (PubMed: 23748078) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kiney)
Specification Kiney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Jun 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3 % Troton X 100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Apr 22 2016

Abreviews
Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney cell line(COS7))
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 30 µg
Specification Kidney cell line(COS7)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (olfactory epithelium)
Specification olfactory epithelium
Fixative Paraformaldehyde
Permeabilization Yes - 0.02% triton X-100
Blocking step blocking reagent from tyramide signal amplification kit (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
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Submitted Apr 29 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"