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Our Abpromise guarantee covers the use of ab17279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. This antibody can be used at 1-10 ug/ml to stain human basic FGF expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4°C, plus 0.1% saponin for 20minutes at room temperature.|
|Sandwich ELISA||Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP1735] to FGF2 (ab92337). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit monoclonal [EP1735] to FGF basic (ab92337) as Detection.|
|ELISA||Use at an assay dependent concentration. This antibody can be used as a coating antibody at 10 µg/ml in a two site ELISA with biotin conjugated bFGF antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human basic FGF in human serum is typically > 85% in the ELISA.|