Overview

  • Product name
    Anti-FGF2 antibody [EP1735]
    See all FGF2 primary antibodies
  • Description
    Rabbit monoclonal [EP1735] to FGF2
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, Sandwich ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human FGF basic.

  • Positive control
    • K562 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AS24] to FGF2 (ab17279). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
    • Tissue specificity
      Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
    • Sequence similarities
      Belongs to the heparin-binding growth factors family.
    • Post-translational
      modifications
      Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
      Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
    • Cellular localization
      Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    Images

    References

    This product has been referenced in:
    • Zhu P  et al. Host Cellular Protein TRAPPC6A? Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. J Virol 91:N/A (2017). Read more (PubMed: 27795429) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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