Overview

  • Product nameAnti-FGF2 antibody [FB-8]
    See all FGF2 primary antibodies
  • Description
    Mouse monoclonal [FB-8] to FGF2
  • SpecificityDoes not cross-react with human fibroblast growth factor - acidic (aFGF).
  • Tested applicationsSuitable for: RIA, ELISA, Dot blot, Neutralising, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab10419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21995307

Target

  • FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

References for Anti-FGF2 antibody [FB-8] (ab10419)

This product has been referenced in:
  • De Benedictis L  et al. Rosiglitazone reverses increased duodenal inhibitory response in spontaneously hypertensive rats. Neurogastroenterol Motil 24:e56-66 (2012). IHC-FoFr ; Rat . Read more (PubMed: 21995307) »
  • Wang L  et al. A novel monoclonal antibody to fibroblast growth factor 2 effectively inhibits growth of hepatocellular carcinoma xenografts. Mol Cancer Ther 11:864-72 (2012). ELISA ; Human . Read more (PubMed: 22351746) »

See all 2 Publications for this product

Product Wall

I have received further information from lab. Unfortunately lab has confirmed that because they do not have any tested data of these antibodies being used as pairs in sELISA and also due to non availability of epitope sequence they are unable to confir...

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Thank you for your email.

We unfortunately have not tested any of these antibodies in sandwich ELISA so we will not be able to confirm their use. Technically, sELISA application needs two antibodies (sELISA pair) which should be raised agains...

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Gracias por contactarnos de nuevo.

¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y gu...

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Thank you for contacting us with your question.

We have 3 different lots in stock and as the concentration varies between these lots, I have created individual documents for each lot. If you require a document for a different lot, please let ...

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Thank you for contacting us. We only provide certificate of compliance for our products. Please find attached the requested certificates. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

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Thank you for your enquiry. Ab10419 is only available unconjugated. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"