Overview

  • Product nameAnti-FGF2 antibody [MC-GF1]
    See all FGF2 primary antibodies
  • Description
    Mouse monoclonal [MC-GF1] to FGF2
  • SpecificityGF1 recognises basic fibroblast growth factor (bFGF).
  • Tested applicationsSuitable for: ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/250. PubMed: 17327226Fix with acetone. Not suitable for detecting bFGF bound to cell surface receptors.

Target

  • FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Anti-FGF2 antibody [MC-GF1] images

  • ab181 staining FGF basic in normal (upper) and diabetic (lower) porcine aortic tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with acetone and air dried for 24 hours. Sections were blocked for endogenous peroxidase activity and incubated with primary antibody at 1/250 dilution. Staining was detected using DAB and counterstained with hematoxylin.

References for Anti-FGF2 antibody [MC-GF1] (ab181)

This product has been referenced in:
  • He W  et al. Blockade of Wnt/ß-catenin signaling by paricalcitol ameliorates proteinuria and kidney injury. J Am Soc Nephrol 22:90-103 (2011). WB ; Mouse . Read more (PubMed: 21030600) »
  • Hsu MY  et al. Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation. Lab Invest 88:842-55 (2008). Read more (PubMed: 18560367) »

See all 4 Publications for this product

Product Wall

Application Flow Cytometry
Sample Mouse Cell (Primary hepatocytes)
Permeabilization Yes - Fluidigm Fix and Perm Buffer
Gating Strategy Positive cells for APC
Specification Primary hepatocytes
Preparation Cell harvesting/tissue preparation method: Liver dissection, followed by mashing the liver to obtain hepatocytes
Sample buffer: PBS + 10% Bovine Serum
Fixation Formaaldehyde
Username

Abcam user community

Verified customer

Submitted May 28 2016

Thank you for your inquiry.
The immunogen used to raise the anti-FGF basic antibody ab181 is the recombinant full length protein(human).
Unfortunately, the epitope of this clone has not been mapped and the pI of our antibodiesare not determined...

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Thank you for your reply.

I have searched this for this PO and the product's order history an cannot find any order using this PO or to Pfizer placed within the last two years. Was this product purchased from an outside distributor?

I...

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Thank you for your reply.

We are very sorry about this. We will happily replace or refund this product for you.

I look forward to your reply on how you like to proceed. Please do not hesitate to contact us if you need any more advic...

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Thank you for your patience as I contacted our lab regarding your inquiry.

I had looked through data regarding this product and found no information to support the statement on the datasheet. These finding were passed onto the production lab ...

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Thank you very much for contacting us.

I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

I am very sorr...

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DISCOUNT CODE: ***
Expiration date: November 6, 2012

This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forab181 and include this code in the “Additional Commen...

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I have received further information from lab. Unfortunately lab has confirmed that because they do not have any tested data of these antibodies being used as pairs in sELISA and also due to non availability of epitope sequence they are unable to confir...

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Thank you for your email.

We unfortunately have not tested any of these antibodies in sandwich ELISA so we will not be able to confirm their use. Technically, sELISA application needs two antibodies (sELISA pair) which should be raised agains...

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Thank you for your enquiry. For use in IHC a suggested working dilution would be 10 ug/ml or 1/100. We would, however, recommend that the end user titrates the antibody around this point to optimise it for their own use.

1-10 of 11 Abreviews or Q&A

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