• Product name
  • Description
    Rabbit polyclonal to FGF21
  • Specificity
    Ab64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB. For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)])
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF21.

    (Peptide available as ab66562.)

  • Positive control
    • This antibody gave a positive signal on Full length FGF-21 Recombinant Protein


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab64857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Recommended dilution / concentration to be published soon
IHC-P Use at an assay dependent concentration. PubMed: 21372207
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).


Anti-FGF21 antibody images

  • Anti-FGF21 antibody (ab64857) at 1 µg/ml + Recombinant human FGF21 protein (ab54141) at 0.1 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.

References for Anti-FGF21 antibody (ab64857)

This product has been referenced in:
  • Dekker Nitert M  et al. Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia. Reprod Biol Endocrinol 13:14 (2015). WB, IHC-P ; Human . Read more (PubMed: 25890271) »
  • Omar BA  et al. Fibroblast growth factor 21 (FGF21) and glucagon-like peptide 1 contribute to diabetes resistance in glucagon receptor-deficient mice. Diabetes 63:101-10 (2014). Mouse . Read more (PubMed: 24062250) »

See all 3 Publications for this product

Product Wall

Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% denaturing gel)
Rat Cell lysate - whole cell (hepatocytes H4IIE cell)
hepatocytes H4IIE cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Cuiqing Zhao

Verified customer

Submitted Jul 22 2013

Thank you for contacting us.

I am happy that this new lot GR85839 gave good results!

With regards to lot GR85321-1, we have 56 units of this lot in stock. If you purchase over 5 units of this antibody we could give you a discount. ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (LIVER LYSATE)
Loading amount
100 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% GRADIENT GEL)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jun 17 2010


Sign up