• Product nameAnti-FGF21 antibody
    See all FGF21 primary antibodies
  • Description
    Rabbit polyclonal to FGF21
  • SpecificityAb64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB. For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)])
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF21.

    (Peptide available as ab66562.)

  • Positive control
    • This antibody gave a positive signal on Full length FGF-21 Recombinant Protein


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab64857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Recommended dilution / concentration to be published soon
IHC-P Use at an assay dependent concentration. PubMed: 21372207
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).


Anti-FGF21 antibody images

  • Anti-FGF21 antibody (ab64857) at 1 µg/ml + Recombinant human FGF21 protein (ab54141) at 0.1 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
    ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.

References for Anti-FGF21 antibody (ab64857)

This product has been referenced in:
  • Omar BA  et al. Fibroblast growth factor 21 (FGF21) and glucagon-like peptide 1 contribute to diabetes resistance in glucagon receptor-deficient mice. Diabetes 63:101-10 (2014). Mouse . Read more (PubMed: 24062250) »
  • Loeffler I  et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). IHC-P ; Mouse . Read more (PubMed: 21372207) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% denaturing gel)
Sample Rat Cell lysate - whole cell (hepatocytes H4IIE cell)
Specification hepatocytes H4IIE cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Cuiqing Zhao

Verified customer

Submitted Jul 22 2013

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (LIVER LYSATE)
Loading amount 100 µg
Specification LIVER LYSATE
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% GRADIENT GEL)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jun 17 2010