Overview

  • Product name
    Anti-FGF21 antibody
    See all FGF21 primary antibodies
  • Description
    Rabbit polyclonal to FGF21
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human FGF21.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab66563.)

  • Positive control
    • This antibody gave a positive signal on Full length FGF-21 Recombinant Protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab66564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    Images

    • Western blot - FGF21 antibody (ab66564)
      Western blot - FGF21 antibody (ab66564)
      Anti-FGF21 antibody (ab66564) at 1 µg/ml + Recombinant human FGF21 protein (ab54141) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 22 kDa
      Observed band size: 24 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



      ab66564 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of ~19.5 kDa.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF21 antibody (ab66564)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF21 antibody (ab66564)
      IHC image of FGF21 staining in Human Liver Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66564, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Jeon JY  et al. Association between insulin resistance and impairment of FGF21 signal transduction in skeletal muscles. Endocrine 53:97-106 (2016). WB . Read more (PubMed: 26758997) »
    • Yang C  et al. Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress. BMC Gastroenterol 13:67 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23590285) »

    See all 2 Publications for this product

    Publishing research using ab66564? Please let us know so that we can cite the reference in this datasheet.

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