Anti-FGF21 antibody (ab66564)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-FGF21 antibody
See all FGF21 primary antibodies -
DescriptionRabbit polyclonal to FGF21
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide derived from within residues 1 - 100 of Human FGF21.
(Peptide available as ab66563.)
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Positive control
- This antibody gave a positive signal on Full length FGF-21 Recombinant Protein (Human)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab66564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. |
Application notes
Is unsuitable for ICC/IF.
Target
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FunctionStimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
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Sequence similaritiesBelongs to the heparin-binding growth factors family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 26291 Human
- Entrez Gene: 56636 Mouse
- Entrez Gene: 170580 Rat
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- SwissProt: Q9JJN1 Mouse
- Unigene: 283015 Human
- Unigene: 143736 Mouse
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Alternative names
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
see all
Anti-FGF21 antibody images
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Western blot - FGF21 antibody (ab66564)Anti-FGF21 antibody (ab66564) at 1 µg/ml +Recombinant human FGF21 protein (ab54141) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF21 antibody (ab66564)IHC image of FGF21 staining in Human Liver Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66564, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-FGF21 antibody (ab66564)
This product has been referenced in:
- Jeon JY et al. Association between insulin resistance and impairment of FGF21 signal transduction in skeletal muscles. Endocrine 53:97-106 (2016). WB . Read more (PubMed: 26758997) »
- Yang C et al. Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress. BMC Gastroenterol 13:67 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23590285) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"