Anti-FGF4 antibody (ab65974)
Key features and details
- Rabbit polyclonal to FGF4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF4 antibody
See all FGF4 primary antibodies -
Description
Rabbit polyclonal to FGF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FGF4 aa 84-97 (internal sequence).
Sequence:RRLYCNVGIGFHLQ
Database link: P08620 -
Positive control
- Hela cell lysis and human lung cancer tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Can transform NIH 3T3 cells from a human stomach tumor (hst) and from karposi's sarcoma (KS3). It has a mitogenic activity. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2249 Human
- Omim: 164980 Human
- SwissProt: P08620 Human
- Unigene: 1755 Human
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Alternative names
- FGF-4 antibody
- Fgf4 antibody
- FGF4_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab65974 has been referenced in 6 publications.
- Landova Sulcova M et al. Developmental mechanisms driving complex tooth shape in reptiles. Dev Dyn 249:441-464 (2020). PubMed: 31762125
- Zhang C et al. miR-511 inhibits proliferation and metastasis of breast cancer cells by targeting FGF4. J Gene Med 22:e3168 (2020). PubMed: 32023352
- Xiao L et al. Cell death, cavitation and spontaneous multi-differentiation of dental pulp stem cells-derived spheroids in vitro: a journey to survival and organogenesis. Biol Cell 106:405-19 (2014). PubMed: 25175801
- Ozawa M et al. The expression of fibroblast growth factor receptors during early bovine conceptus development and pharmacological analysis of their actions on trophoblast growth in vitro. Reproduction 145:191-201 (2013). PubMed: 23241344
- Xiao L & Tsutsui T Characterization of human dental pulp cells-derived spheroids in serum-free medium: stem cells in the core. J Cell Biochem 114:2624-36 (2013). PubMed: 23794488
- Dobrowolski R et al. Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice. Hum Mol Genet 18:2899-911 (2009). PubMed: 19439426