Overview

  • Product nameFGF4 Human ELISA KitSee all FGF4 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 50 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    > 90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91.54 80% - 103%
    Serum 97.57 89% - 112%
    Plasma 96.21 88% - 110%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s FGF4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF4 in serum, plasma (Human FGF4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), and cell culture supernatants.

    This assay employs an antibody specific for Human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceFGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.
  • Alternative names
    • Fibroblast growth factor 4
    • HBGF4
    • Heparin binding growth factor 4
    • Heparin secretory transforming protein 1
    • HST
    • HST1
    • HSTF1
    • KS3
    • Transforming protein KS3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

FGF4 Human ELISA Kit images

  • Representative Standard Curve using ab100516

  • Representative Standard Curve using ab100516

Protocols

References for FGF4 Human ELISA Kit (ab100516)

ab100516 has not yet been referenced specifically in any publications.

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