Overview

  • Product name
  • Description
    Rabbit polyclonal to FGF5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rabbit, Cow, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF5.

  • Positive control
    • This anitbody gave a positive signal in the following tissues lysates: E14 Mouse Embryo Brain; E16 Ms Embryo Brain; E18 Ms Embryo Brain This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.

Properties

Applications

Our Abpromise guarantee covers the use of ab88118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
  • Tissue specificity
    Expressed in neonatal brain.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Developmental stage
    Can transform NIH 3T3 cells.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGF 5 antibody
    • FGF-5 antibody
    • FGF5 antibody
    • FGF5_HUMAN antibody
    • Fibroblast growth factor 5 antibody
    • HBGF 5 antibody
    • HBGF-5 antibody
    • heparin binding growth factor 5 antibody
    • Heparin-binding growth factor 5 antibody
    • Smag 82 antibody
    • Smag-82 antibody
    • TCMGLY antibody
    see all

Images

  • ICC/IF image of ab88118 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88118 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E16 Ms Embryo Brain Tissue Lysate
    Lane 3 : E18 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References

This product has been referenced in:
  • Ding BS  et al. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci 128:2983-8 (2015). IHC ; Human . Read more (PubMed: 26116571) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab88118.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up