This anitbody gave a positive signal in the following tissues lysates:
E14 Mouse Embryo Brain; E16 Ms Embryo Brain; E18 Ms Embryo Brain
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ICC/IF image of ab88118 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88118 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-FGF5 antibody (ab88118)
All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate Lane 2 : E16 Ms Embryo Brain Tissue Lysate Lane 3 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa Observed band size: 30 kDa Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.