Overview

  • Product nameFGF7 Human ELISA Kit
    See all FGF7 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.5 pg/ml
  • Range
    0.55 pg/ml - 400 pg/ml
  • Recovery

    > 93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.24 81% - 98%
    Serum 112.9 102% - 123%
    Plasma 126.2 114% - 135%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s FGF7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human FGF7 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceFibroblast growth factors (FGFs) can stimulate the proliferation of mesenchymal, epithelial and neuroectodermal cells. Members of the family include Acidic FGF (also known as aFGF or FGF 1), basic FGF (bFGF or FGF 2), FGF3 (also known as Int2) and FGF4 (also known as hst or Kaposi FGF). FGF4 was first revealed to be an oncoprotein in Kaposi's sarcoma cells. Other family members include FGF5, FGF6, FGF7 (keratinocyte growth factor, KGF), FGF8 (AIGF), FGF9 (GAF) and FGF10.
  • Cellular localizationSecreted
  • Alternative names
    • FGF 7
    • Fibroblast growth factor 7
    • HBGF 7
    • Heparin binding growth factor 7
    • Keratinocyte growth factor
    • KGF
    • OTTMUSP00000016341
    • OTTMUSP00000016342
    • RP23-386I21.3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

FGF7 Human ELISA Kit images

  • Representative Standard Curve using ab100519

Protocols

References for FGF7 Human ELISA Kit (ab100519)

ab100519 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"