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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF9.
(Peptide available as ab86940.)
Our Abpromise guarantee covers the use of ab71395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 23 kDa).|
ab71395 staining FGF9 in monkey fetal lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.