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Our Abpromise guarantee covers the use of ab9743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution. PubMed: 17192470|
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9 (using 100µl/well antibody solution).|
|Neutralising||Use a concentration of 0.025 - 0.06 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.5 ng/ml).|
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