Overview

  • Product nameAnti-FGF9 antibody
    See all FGF9 primary antibodies
  • Description
    Rabbit polyclonal to FGF9
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Highly pure (>98%) recombinant mFGF-9 (mouse Fibroblast Growth Factor-9).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 17192470
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9 (using 100µl/well antibody solution).
Neutralising Use a concentration of 0.025 - 0.06 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.5 ng/ml).

Target

  • FunctionMay have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
  • Tissue specificityGlial cells.
  • Involvement in diseaseDefects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGF 9 antibody
    • FGF-9 antibody
    • FGF9 antibody
    • FGF9_HUMAN antibody
    • Fibroblast growth factor 9 antibody
    • GAF antibody
    • Glia Activating Factor antibody
    • Glia-activating factor antibody
    • HBFG 9 antibody
    • HBFG9 antibody
    • HBGF-9 antibody
    • Heparin-binding growth factor 9 antibody
    • MGC119914 antibody
    • MGC119915 antibody
    • SYNS3 antibody
    see all

Anti-FGF9 antibody images

  • All lanes : Anti-FGF9 antibody (ab9743) at 1/500 dilution

    Lane 1 : Recombinant FGF9
    Lane 2 : Rat cerebral cortex membrane whole tissue lysate. at 15 µg

    Secondary
    Mouse anti-rabbit IgG monoclonal at 1/1000 dilution
    Developed using the ECL technique

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab9743 staining FGF9 in mouse lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References for Anti-FGF9 antibody (ab9743)

This product has been referenced in:
  • Arnaud-Dabernat S  et al. FGFR3 contributes to intestinal crypt cell growth arrest. J Cell Physiol 216:261-8 (2008). Read more (PubMed: 18286540) »
  • Arnaud-Dabernat S  et al. FGFR3 is a negative regulator of the expansion of pancreatic epithelial cells. Diabetes 56:96-106 (2007). IHC-P ; Mouse . Read more (PubMed: 17192470) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lymph node)
Specification Lymph node
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

Submitted Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (brain)
Loading amount 15 µg
Specification brain
Gel Running Conditions Reduced Denaturing (gel 15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 16°C
Username

Abcam user community

Verified customer

Submitted Apr 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"