Overview

  • Product nameFGF9 Human ELISA KitSee all FGF9 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 200 pg/ml
  • Range
    358.4 pg/ml - 35000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.27 88% - 107%
    Serum 97.52 85% - 105%
    Plasma 94.38 83% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s FGF9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF9 in serum, plasma (Human FGF9 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), and cell culture supernatants.

    This assay employs an antibody specific for Human FGF9 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionPlays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
  • Tissue specificityGlial cells.
  • Involvement in diseaseDefects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P31371 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • Elbow knee synostosis
    • FGF 9
    • FGF-9
    • FGF9
    • FGF9_HUMAN
    • Fibroblast growth factor 9
    • GAF
    • Glia Activating Factor
    • Glia-activating factor
    • HBFG 9
    • HBFG9
    • HBGF-9
    • Heparin binding growth factor 9
    • Heparin-binding growth factor 9
    • MGC119914
    • MGC119915
    • SYNS3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

FGF9 Human ELISA Kit images

  • Representative Standard Curve using ab100520.

  • Representative Standard Curve using ab100520.

Protocols

References for FGF9 Human ELISA Kit (ab100520)

ab100520 has not yet been referenced specifically in any publications.

Product Wall

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply. The microplate is from Greiner, cat# 762071. This should allow you to find any specific dimension information needed. Please let me know if you have any further questions.

Thank you for contacting us.

These kits are provided with 12 strips of 8 wells each in a frame that is the same size as a standard 96-well plate (˜5 x 3.33"). If you need any more specific information, please let me know and I will be happy t...

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Thank you for contacting Abcam earlier today.

I have spoken to the lab and they have advised that you should not store the detection antibody concentrate for longer than 5 days at 4ºC. While not ideal, if you absolutely need to store it ...

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