The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWestern blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1.
Immunoprecipitation: Ig loop I fragment of denatured, intact FGFr1a.
Immunohistochemistry: untested; predicted to be poor due to masked epitope.
RelevanceFGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.