Overview

  • Product nameAnti-FGFR1 alpha antibody [M2F12]
    See all FGFR1 alpha primary antibodies
  • Description
    Mouse monoclonal [M2F12] to FGFR1 alpha
  • SpecificityReacts with alpha isoform of FRFR1.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

  • EpitopeNH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.
  • Positive control
    • IHC: Rat prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19728793
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa.

Target

  • RelevanceFGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • BFGFR antibody
    • CD331 antibody
    • CEK antibody
    • FGFBR antibody
    • FGFR1 antibody
    • FLG antibody
    • FLT2 antibody
    • FMS like tyrosine kinase 2 antibody
    • HBGFR antibody
    • KAL2 antibody
    • N SAM antibody
    • OGD antibody
    see all

Anti-FGFR1 alpha antibody [M2F12] images

  • ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FGFR1 alpha antibody [M2F12] (ab829)

This product has been referenced in:
  • Hellingman CA  et al. FIBROBLAST GROWTH FACTOR RECEPTORS IN IN-VITRO AND IN-VIVO CHONDROGENESIS: RELATING TISSUE ENGINEERING USING ADULT MESENCHYMAL STEM CELLS TO EMBRYONIC DEVELOPMENT. Tissue Eng Part A : (2009). IHC-P ; Mouse . Read more (PubMed: 19728793) »
  • Tsui TY  et al. rAAV-mediated stable expression of heme oxygenase-1 in stellate cells: a new approach to attenuate liver fibrosis in rats. Hepatology 42:335-42 (2005). IHC-Fr ; Mouse . Read more (PubMed: 16025519) »

See all 5 Publications for this product

Product Wall

No - this antibody is specific for FGFR1.

Ab829 binds on the cell surface.

Thank you for your patience. I have been discussing this complaint with my colleagues. Unfortunately, we do not really know why this antibody did not perform well for you. We haven't had any complaints about this one. In fact, most of our custome...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"