Anti-FGFR1OP2 antibody (ab229119)
Key features and details
- Rabbit polyclonal to FGFR1OP2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FGFR1OP2 antibody -
Description
Rabbit polyclonal to FGFR1OP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment within Human FGFR1OP2 (internal sequence). The exact sequence is proprietary.
Database link: Q9NVK5 -
Positive control
- WB: MCF7 whole cell lysate; mouse stomach tissue lysate. IHC-P: H441 xenograft tissue. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
Target
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Function
May be involved in wound healing pathway. -
Tissue specificity
Expressed in bone marrow, spleen and thymus. -
Involvement in disease
Note=A chromosomal aberration involving FGFR1OP2 may be a cause of stem cell myeloproliferative disorder (MPD). Insertion ins(12;8)(p11;p11p22) with FGFR1. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein FGFR1OP2-FGFR1 may exhibit constitutive kinase activity and be responsible for the transforming activity. -
Sequence similarities
Belongs to the SIKE family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 26127 Human
- Entrez Gene: 67529 Mouse
- Entrez Gene: 362463 Rat
- Omim: 608858 Human
- SwissProt: Q9NVK5 Human
- SwissProt: Q9CRA9 Mouse
- SwissProt: Q6TA25 Rat
- Unigene: 591162 Human
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Alternative names
- DKFZp564O1863 antibody
- FGFR1 oncogene partner 2 antibody
- FGFR1OP2 antibody
see all
Images
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Anti-FGFR1OP2 antibody (ab229119) at 1/2000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 29 kDa12% SDS-PAGE
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Anti-FGFR1OP2 antibody (ab229119) at 1/1000 dilution + Mouse stomach tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa12% SDS-PAGE
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Paraffin-embedded H441 xenograft tissue stained for FGFR1OP2 with ab229119 at 1/500 dilution in immunohistochemical analysis.
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Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for FGFR1OP2 (green) using ab229119 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229119 has not yet been referenced specifically in any publications.