Overview

  • Product nameAnti-FGFR2 antibody
    See all FGFR2 primary antibodies
  • Description
    Rabbit polyclonal to FGFR2
  • SpecificityFGFR2, Cytoplasmic reacts specifically with FGFR2 using immunoblotting and immunoprecipitation (doublet at 115-125kD) using whole cell lysates of transfected 293T cells, a human, embryonic kidney cell line, expressing recombinant human FGFR2. No reaction with human FGFR1 and FGFR3 is detected.
  • Tested applicationsSuitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KLPQYPHINGSVKT

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 809-821 of the cytoplasmic region of Human FGFR2.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab10647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
IHC-P
  • Application notesIHC-P: Use at an assay dependent dilution, this concentration is determined by indirect immunoperoxidase staining of trypsin or protease-digested human and animal tissue sections.
    IP: 1/2000. This concentration is determined by immunoprecipitation using a whole lysate of transfected cells expressing recombinant human FGFR2.
    WB: 1/2000. This concentration is determined by immunoblotting using a whole extract of transfected cells expressing recombinant human FGFR2. Predicted molecular weight: 110 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for acidic and basic fibroblast growth factors.
    • Involvement in diseaseDefects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
      Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
      Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
      Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
      Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
      Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
      Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
      Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • bacteria-expressed kinase antibody
      • BBDS antibody
      • BEK antibody
      • BEK fibroblast growth factor receptor antibody
      • BFR1 antibody
      • CD332 antibody
      • CD332 antigen antibody
      • CEK3 antibody
      • CFD1 antibody
      • Craniofacial dysostosis 1 antibody
      • ECT1 antibody
      • FGF receptor antibody
      • FGFR 2 antibody
      • FGFR-2 antibody
      • Fgfr2 antibody
      • FGFR2_HUMAN antibody
      • Fibroblast growth factor receptor 2 antibody
      • Hydroxyaryl protein kinase antibody
      • Jackson Weiss syndrome antibody
      • JWS antibody
      • K SAM antibody
      • K-sam antibody
      • Keratinocyte growth factor receptor 2 antibody
      • Keratinocyte growth factor receptor antibody
      • KGFR antibody
      • KSAM antibody
      • protein tyrosine kinase, receptor like 14 antibody
      • soluble FGFR4 variant 4 antibody
      • TK14 antibody
      • TK25 antibody
      see all

    References for Anti-FGFR2 antibody (ab10647)

    This product has been referenced in:
    • Spence JR  et al. Retina regeneration in the chick embryo is not induced by spontaneous Mitf downregulation but requires FGF/FGFR/MEK/Erk dependent upregulation of Pax6. Mol Vis 13:57-65 (2007). Read more (PubMed: 17277739) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. I have confirmed that the discrepancy is indeed a typographical error. I have updated the datasheet with the correct sequence. I hope this information helps. Please do not hesitate to contact us if you need anything f...

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    Thank you for your enquiry. Further to correspondence with the source of this antibody unfortunately I have been unable to determine whether the multiple bands that you have been observing is typical of any of these antibodies. As a general rule...

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    I was able to find out what protocol was used on transfected 293T cells expressing recombinant human FGFR2: 1. Extract: 0.5 plate/slab of transfected 293T cells expressing recombinant human FGFR-2. 7-10% gel. The proteins were run on a reducing gel....

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    Thank you very much for taking the time to send me the photograph of your blots and for the extra information, it is very useful. The source has no reported problems with this antibody. The size range of FGFR2 receptor should be between 115 and 125KD...

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    Thank you very much for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused. We do not routine...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"