Overview

  • Product name
  • Description
    Rabbit polyclonal to FGFRL1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 150 - 486 of Human FGFRL1

  • Positive control
    • WB: 293T, HepG2, and Raji cell lysates IHC-P: Oral CA Cal27 tissue This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/250.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Has a negative effect on cell proliferation.
  • Tissue specificity
    Expressed preferentially in cartilaginous tissues and pancreas. Highly expressed in the liver, kidney, heart, brain and skeletal muscle. Weakly expressed in the lung, small intestine and spleen.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Membrane. Predominantly localized in the plasma membrane but also detected in the Golgi and in secretory vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FGF homologous factor receptor antibody
    • FGF receptor like protein 1 antibody
    • FGF receptor-like protein 1 antibody
    • FGFR 5 antibody
    • FGFR like protein antibody
    • FGFR-5 antibody
    • FGFR-like protein antibody
    • FGFR5 antibody
    • Fgfrl1 antibody
    • FGRL1_HUMAN antibody
    • FHFR antibody
    • Fibroblast growth factor receptor 5 antibody
    • Fibroblast growth factor receptor like 1 antibody
    • Fibroblast growth factor receptor-like 1 antibody
    see all

Images

  • ICC/IF image of ab95940 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95940 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-FGFRL1 antibody (ab95940) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 55 kDa
    10% SDS PAGE
  • ab95940, at a 1/100 dilution, staining FGFRL1 in paraffin-embedded Oral CA Cal27 by Immunohistochemical analysis.

References

This product has been referenced in:
  • Siegfried JM  et al. Interaction between the estrogen receptor and fibroblast growth factor receptor pathways in non-small cell lung cancer. Oncotarget 8:24063-24076 (2017). WB . Read more (PubMed: 28445992) »
  • di Martino E  et al. An integrated genomic, transcriptional and protein investigation of FGFRL1 as a putative 4p16.3 deletion target in bladder cancer. Genes Chromosomes Cancer N/A:N/A (2013). Read more (PubMed: 23775577) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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