Overview

  • Product nameAnti-FGGY antibody
    See all FGGY primary antibodies
  • Description
    Mouse polyclonal to FGGY
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length FGGY carbohydrate kinase domain containing protein (Human)

  • Positive control
    • FGGY carbohydrate kinase domain containing transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 40 kDa, which is similar to that expected for isoform 3 (predicted molecular weight: 60 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityExpressed in kidney, lung and small intestine and to a lower extent in liver and detected in cerebrospinal fluid (at protein level).
    • Involvement in diseaseDefects in FGGY are associated with sporadic amyotrophic lateral sclerosis (ALS) [MIM:105400]. Amyotrophic lateral sclerosis is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors.
    • Sequence similaritiesBelongs to the FGGY kinase family.
    • Developmental stageExpressed in fetal brain (at protein level).
    • Information by UniProt
    • Database links
    • Alternative names
      • fggy antibody
      • FGGY carbohydrate kinase domain containing antibody
      • FGGY carbohydrate kinase domain-containing protein antibody
      • FGGY_HUMAN antibody
      • FLJ10986 antibody
      • MGC94804 antibody
      • OTTHUMP00000010078 antibody
      • OTTHUMP00000010081 antibody
      • OTTHUMP00000010082 antibody
      • OTTHUMP00000202071 antibody
      • RP11-242B9.1 antibody
      see all

    Anti-FGGY antibody images

    • All lanes : Anti-FGGY antibody (ab67835) at 1/500 dilution

      Lane 1 : FGGY carbohydrate kinase domain containing transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 60 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    References for Anti-FGGY antibody (ab67835)

    ab67835 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - other (MEF, C2C12)
    Loading amount 25 µg
    Specification MEF, C2C12
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (hepG2, 293T)
    Loading amount 25 µg
    Specification hepG2, 293T
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 24 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"