Overview

  • Product nameAnti-FGGY antibody
    See all FGGY primary antibodies
  • Description
    Mouse monoclonal to FGGY
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment: MWLDHRAVSQ VNRINETKHS VLQYVGGVMS VEMQAPKLLW LKENLREICW DKAGHFFDLP DFLSWKATGV TARSLCSLVC KWTYSAEKGW DDSFWKMIG, corresponding to amino acids 113-211 of Human FGGY carbohydrate kinase domain containing (NP_060761) with proprietary tag.

  • Positive control
    • HeLa cell lysate; Recombinant tagged human FGGY carbohydrate kinase domain containing fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Tissue specificityExpressed in kidney, lung and small intestine and to a lower extent in liver and detected in cerebrospinal fluid (at protein level).
  • Involvement in diseaseDefects in FGGY are associated with sporadic amyotrophic lateral sclerosis (ALS) [MIM:105400]. Amyotrophic lateral sclerosis is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors.
  • Sequence similaritiesBelongs to the FGGY kinase family.
  • Developmental stageExpressed in fetal brain (at protein level).
  • Information by UniProt
  • Database links
  • Alternative names
    • fggy antibody
    • FGGY carbohydrate kinase domain containing antibody
    • FGGY carbohydrate kinase domain-containing protein antibody
    • FGGY_HUMAN antibody
    • FLJ10986 antibody
    • MGC94804 antibody
    • OTTHUMP00000010078 antibody
    • OTTHUMP00000010081 antibody
    • OTTHUMP00000010082 antibody
    • OTTHUMP00000202071 antibody
    • RP11-242B9.1 antibody
    see all

Anti-FGGY antibody images

  • Anti-FGGY antibody (ab77723) at 1 µg/ml + Recombinant tagged human FGGY carbohydrate kinase domain containing fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-FGGY antibody (ab77723) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-FGGY antibody (ab77723)

ab77723 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T)
Loading amount 25 µg
Specification HepG2, 293T
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Aug 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (MEF, C2C12)
Loading amount 25 µg
Specification MEF, C2C12
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"