Overview

  • Product nameAnti-FGL2 antibody
    See all FGL2 primary antibodies
  • Description
    Mouse monoclonal to FGL2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Recombinant Fragment
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment: NNETEEIKDE RAKDVCPVRL ESRGKCEEAG ECPYQVSLPP LTIQLPKQFS RIEEVFKEVQ NLKEIVNSLK KSCQDCKLQA DDNGDPGRNG LLLPSTGAPG , corresponding to amino acids 24-124 of Human FGL2, NP_006673, with a 26kDa tag.

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
ELISA Use at an assay dependent dilution.

Target

Anti-FGL2 antibody images

  • Anti-FGL2 antibody (ab77642) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-FGL2 antibody (ab77642) at 5 µg/ml + Hela cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-FGL2 antibody (ab77642)

ab77642 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"