Overview

  • Product nameAnti-FH antibody
    See all FH primary antibodies
  • Description
    Goat polyclonal to FH
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Drosophila melanogaster, Zebrafish, a wide range of other species, Cynomolgus Monkey, Chlamydia trachomatis, Orangutan
  • Immunogen

    Synthetic peptide:

    C-HPNDHVNKSQSSND

    , corresponding to internal sequence amino acids 176-189 of Human FH

  • Positive control
    • Human Kidney, Liver and Lung lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab62825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect recombinant Fumarase
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 55 kDa).
ELISA 1/64000.

Target

  • FunctionAlso acts as a tumor suppressor.
  • PathwayCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
  • Involvement in diseaseDefects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
    Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
    Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
  • Sequence similaritiesBelongs to the class-II fumarase/aspartase family. Fumarase subfamily.
  • Cellular localizationCytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FH antibody
    • Fumarase antibody
    • Fumarate hydratase antibody
    • Fumarate hydratase mitochondrial antibody
    • Fumarate hydratase, mitochondrial antibody
    • FUMH_HUMAN antibody
    • HLRCC antibody
    • LRCC antibody
    • MCL antibody
    • MCUL 1 antibody
    • MCUL1 antibody
    • MS709 antibody
    • Multiple hereditary cutaneous leiomyomata antibody
    see all

Anti-FH antibody images

  • Anti-FH antibody (ab62825) at 0.01 µg/ml + Human Kidney lysate in RIPA buffer at 35 µg/ml

    Predicted band size : 55 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


  • Predicted band size : 55 kDa

References for Anti-FH antibody (ab62825)

This product has been referenced in:
  • Kienhöfer J  et al. Association of mitochondrial antioxidant enzymes with mitochondrial DNA as integral nucleoid constituents. FASEB J 23:2034-44 (2009). WB ; Human . Read more (PubMed: 19228881) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"