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Product name Anti-FH antibody See all FH primary antibodies
Rabbit polyclonal to FH
Tested applications Suitable for:
WB, IHC-P, ICC/IF more details
Predicted to work with:
Mouse, Rat, Cow, Zebrafish
Recombinant fragment containing a sequence corresponding to a region within amino acids 195-443 of Human FH (NP_000134).
HeLa and HepG2 whole cell lysates; HeLa cells; NCIN87 xenograft; 293T, A431, H1299, Molt, and Raji cell lines
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
1/100 - 1/500.
1/100 - 1/200.
Function Also acts as a tumor suppressor.
Pathway Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Involvement in disease Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia. Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids). Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
Sequence similarities Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Cellular localization Cytoplasm and Mitochondrion.
Information by UniProt
Fumarate hydratase antibody
Anti-FH antibody images
Western blot - FH antibody (ab95947)
All lanes : Anti-FH antibody (ab95947) at 1/1000 dilution Lane 1 : HeLa whole cell lysate Lane 2 : HepG2 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FH antibody (ab95947)
Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, using ab95947 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - FH antibody (ab95947)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, using ab95947 at 1/200 dilution. Right image is merged with DNA probe.
References for Anti-FH antibody (ab95947)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"