Recombinant
RabMAb

Anti-FH antibody [EPR11648] (ab171948)

Overview

  • Product name
    Anti-FH antibody [EPR11648]
    See all FH primary antibodies
  • Description
    Rabbit monoclonal [EPR11648] to FH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P07954

  • Positive control
    • HepG2 cells and HepG2, HeLa, 293T and A459 cell lysates,
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Also acts as a tumor suppressor.
    • Pathway
      Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
    • Involvement in disease
      Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
      Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
      Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
    • Sequence similarities
      Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
    • Cellular localization
      Cytoplasm and Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FH antibody
      • Fumarase antibody
      • Fumarate hydratase antibody
      • Fumarate hydratase mitochondrial antibody
      • Fumarate hydratase, mitochondrial antibody
      • FUMH_HUMAN antibody
      • HLRCC antibody
      • LRCC antibody
      • MCL antibody
      • MCUL 1 antibody
      • MCUL1 antibody
      • MS709 antibody
      • Multiple hereditary cutaneous leiomyomata antibody
      see all

    Images

    • Immunofluorescent analysis of HepG2 cells labeling FH with ab171948 (green) and DAPI nuclear staining (blue).

    • All lanes : Anti-FH antibody [EPR11648] (ab171948) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    References

    ab171948 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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