Anti-FHIT antibody (ab53074)


  • Product name
  • Description
    Rabbit polyclonal to FHIT
  • Specificity
    FHIT antibody detects endogenous levels of total FHIT protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human FHIT

  • Positive control
    • Human breast carcinoma; A549 cell extract.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab53074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.


  • Relevance
    FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
  • Cellular localization
  • Database links
  • Alternative names
    • AP3A hydrolase antibody
    • AP3A hydrolase fragile site 3p14.2 antibody
    • AP3Aase antibody
    • Bis 5' adenosyl triphosphatase antibody
    • Bis(5' adenosyl) triphosphatase antibody
    • Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody
    • Dinucleosidetriphosphatase antibody
    • FRA 3B antibody
    • FRA3B antibody
    • Fragile histidine triad antibody
    • Fragile histidine triad gene antibody
    • Fragile histidine triad protein antibody
    • Tumor suppressor protein antibody
    see all


  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53074 at 1/50 dilution, with and without peptide immunogen.
  • All lanes : Anti-FHIT antibody (ab53074) at 1/500 dilution

    Lane 1 : A549 cell extract with no immunizing peptide
    Lane 2 : A549 cell extract with immunizing peptide

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
  • ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab53074 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply.

I am pleased to provide the testing protocol below, including the antigen retreival. I hope this will be helpful. Please note this is a guideline only, and may require some further individual optimization by the end...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (lymphocyte)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Julian Laubental

Verified customer

Submitted Apr 20 2012

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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Thank you for your patient. The immunogen peptide sequence range is amino acids 110-130. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


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