Anti-FHIT (phospho Y114) antibody (ab111557)

Overview

  • Product nameAnti-FHIT (phospho Y114) antibody
    See all FHIT primary antibodies
  • Description
    Rabbit polyclonal to FHIT (phospho Y114)
  • Specificityab111557 detects endogenous levels of FHIT only when phosphorylated at tyrosine 114.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from Human FHIT around the phosphorylation site of Tyrosine 114 (S-I-YP-E-E).

  • Positive control
    • Human colon carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111557 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceFHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • AP3A hydrolase antibody
    • AP3A hydrolase fragile site 3p14.2 antibody
    • AP3Aase antibody
    • Bis 5' adenosyl triphosphatase antibody
    • Bis(5' adenosyl) triphosphatase antibody
    • Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody
    • Dinucleosidetriphosphatase antibody
    • FRA 3B antibody
    • FRA3B antibody
    • Fragile histidine triad antibody
    • Fragile histidine triad gene antibody
    • Fragile histidine triad protein antibody
    • Tumor suppressor protein antibody
    see all

Anti-FHIT (phospho Y114) antibody images

  • ab111557, at 1/50 dilution, staining FHIT in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-FHIT (phospho Y114) antibody (ab111557)

ab111557 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"