Overview

  • Product name
  • Description
    Rabbit polyclonal to FHL2
  • Specificity
    Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.

  • Positive control
    • HeLa nuclear extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is affinity purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ICC/IF 1/100.

Target

  • Function
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • Tissue specificity
    Expressed in skeletal muscle and heart.
  • Sequence similarities
    Contains 4 LIM zinc-binding domains.
  • Domain
    The third LIM zinc-binding mediates interaction with E4F1.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Anti-FHL2 antibody images



  • Predicted band size : 32 kDa


    Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
  • ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FHL2 antibody (ab12327)

This product has been referenced in:
  • Xue YL  et al. Plumbagin exhibits an anti-proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/ß-catenin signalling. Oncol Lett 12:1095-1100 (2016). WB ; Human . Read more (PubMed: 27446400) »
  • Friedrich FW  et al. FHL2 expression and variants in hypertrophic cardiomyopathy. Basic Res Cardiol 109:451 (2014). Read more (PubMed: 25358972) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization
Yes - saponin
Specification
Human pluripotent stem cell derived cardiomyocyte
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Mouse Cell lysate - whole cell (bone marrow cells)
Specification
bone marrow cells
Treatment
30 ng/ml RANKL and 30 ng/ml MCSF for 5 days
Total protein in input
2000 µg
Username

Do Yu Soung

Verified customer

Submitted Jul 26 2013

Le peptide bloquant de l'anti-FHL2 ab12327 est maintenant disponible à la vente sous la référence ab119360. La fiche technique est disponible via le lien www.abcam.com/ab119360 . J'espère que ces informations vous seront utiles. N'hésitez pas à nous...

Read More

Le laboratoire m'a informé que le peptide bloquant est disponible à la vente, il sera ajouté au catalogue dans la journée d'aujourd'hui ou au plus tard demain. Je vous enverrai la référence de ce produit quand la fiche technique aura été créée. La s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (osteoblast)
Specification
osteoblast
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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