Overview

  • Product nameAnti-FHL2 antibody
    See all FHL2 primary antibodies
  • Description
    Rabbit polyclonal to FHL2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: YAKKCAGCTN PISGLGGTKY ISFEERQWHN DCFNCKKCSL SLVGRGFLTE, corresponding to a region within C terminal amino acids 217-266 of Human FHL2

  • Positive control
    • Myocardium

Properties

Applications

Our Abpromise guarantee covers the use of ab66399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionMay function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • Tissue specificityExpressed in skeletal muscle and heart.
  • Sequence similaritiesContains 4 LIM zinc-binding domains.
  • DomainThe third LIM zinc-binding mediates interaction with E4F1.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Anti-FHL2 antibody images

  • Anti-FHL2 antibody (ab66399) at 0.6 µg/ml + Fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • ab66399, at 8µg/ml, staining Human FHL2 in myocardial cells (indicated with arrows) in paraffin-embedded heart tissue, by Immunohistochemistry, 400x magnification.

References for Anti-FHL2 antibody (ab66399)

This product has been referenced in:
  • Matulis CK & Mayo KE The LIM domain protein FHL2 interacts with the NR5A family of nuclear receptors and CREB to activate the inhibin-a subunit gene in ovarian granulosa cells. Mol Endocrinol 26:1278-90 (2012). Human . Read more (PubMed: 22734036) »
  • Isumi Y  et al. Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice. Biochem Biophys Res Commun 405:216-21 (2011). WB ; Human . Read more (PubMed: 21219870) »

See all 2 Publications for this product

Product Wall

Merci de nous avoir contactés.

Le laboratoire qui produit l'anticorps ab66399 m'a confirmé que le démasquage de l'antigène en IHC-P a été effectuéà 98-100°C, en micro-ondes,avec un ta...

Read More

Thank you for contacting us and for your interest in our products. If you want to use the antibody for immunofluorescence on cell culture samples (ICC-IF, immunocytochemistry), I am sorry to confirm that as far as we are aware, ab66399 and ab1...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"