Overview

  • Product name
  • Description
    Mouse polyclonal to FHL3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Full-length protein corresponding to amino acids 1-280 of Human FHL3 (NP_004459.2).

  • Positive control
    • Lysate from FHL3 transfected 293T cells; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab168787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 10 µg/ml. For IF applications, antibody purification with Protein A will be needed prior to use.

Target

Images

  • All lanes : Anti-FHL3 antibody (ab168787) at 1/500 dilution

    Lane 1 : FHL3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.


    Predicted band size : 31 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FHL3 using ab168787 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining. ab168787 should be Protein A purified before use.

References

ab168787 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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