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Mouse polyclonal to FHL3
Predicted to work with:
Full-length protein corresponding to amino acids 1-280 of Human FHL3 (NP_004459.2).
Lysate from FHL3 transfected 293T cells; HeLa cells
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
Use a concentration of 10 µg/ml. For IF applications, antibody purification with Protein A will be needed prior to use.
Expressed only in skeletal muscle.
Contains 4 LIM zinc-binding domains.
Information by UniProt
FHL 3 antibody
Western blot - Anti-FHL3 antibody (ab168787)
All lanes : Anti-FHL3 antibody (ab168787) at 1/500 dilution Lane 1 : FHL3 transfected 293T cell lysate Lane 2 : Non transfected 293T cell lysate Lysates/proteins at 15 µl per lane. Predicted band size : 31 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody (ab168787)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FHL3 using ab168787 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining. ab168787 should be Protein A purified before use.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"