Recombinant Anti-FHL3 antibody [EPR14243(B)] (ab180509)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14243(B)] to FHL3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FHL3 antibody [EPR14243(B)]
See all FHL3 primary antibodies -
Description
Rabbit monoclonal [EPR14243(B)] to FHL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle, K562 cell line & Rat muscle lysates, Human muscle tissue, A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14243(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Tissue specificity
Expressed only in skeletal muscle. -
Sequence similarities
Contains 4 LIM zinc-binding domains. - Information by UniProt
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Database links
- Entrez Gene: 2275 Human
- Entrez Gene: 14201 Mouse
- Entrez Gene: 313582 Rat
- Omim: 602790 Human
- SwissProt: Q13643 Human
- SwissProt: Q9R059 Mouse
- Unigene: 57687 Human
- Unigene: 331856 Mouse
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Alternative names
- FHL 3 antibody
- FHL-3 antibody
- Fhl3 antibody
see all
Images
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All lanes : Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Rat muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 31 kDaBlocking buffer: 5% NFDM/TBST
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Immunofluorescence analysis of A673 cells labeling FHL3 with ab180509 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/250 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
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Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling FHL3 with ab180509 at 1/500 dilution. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/5000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG HRP (ab136636) at 1/500 dilution
Predicted band size: 31 kDaBlocking buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180509 has not yet been referenced specifically in any publications.