Overview

  • Product name
  • Description
    Mouse polyclonal to FHOD1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    full-length human FHOD1 protein (NP_037373.2, 1 a.a. ~ 1164 a.a).

  • Positive control
    • K-562 cell lysate and FHOD1 transfected 293T cell lysate human spleen

Properties

Applications

Our Abpromise guarantee covers the use of ab73443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 127 kDa.
IHC-P Use a concentration of 13 µg/ml.

Target

  • Function
    Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.
  • Tissue specificity
    Ubiquitous. Highly expressed in spleen.
  • Sequence similarities
    Belongs to the formin homology family.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Domain
    Regulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • FH1/FH2 domain containing protein antibody
    • FH1/FH2 domain containing protein 1 antibody
    • FH1/FH2 domain-containing protein 1 antibody
    • Fhod1 antibody
    • FHOD1_HUMAN antibody
    • FHOS antibody
    • FHOS1 antibody
    • Formin homolog overexpressed in spleen 1 antibody
    • Formin homology 2 domain containing 1 antibody
    • Formin homology 2 domain containing protein 1 antibody
    • Formin homology 2 domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-FHOD1 antibody (ab73443) at 1/500 dilution

    Lane 1 : FHOD1 transfected 293T cell lysate
    Lane 2 : non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 127 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • Anti-FHOD1 antibody (ab73443) at 1/500 dilution + K-562 cell lysate at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 127 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.

References

This product has been referenced in:

See all 3 Publications for this product

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