Anti-FHOD1 (phospho T1141) antibody (ab157589)

Overview

  • Product name
    Anti-FHOD1 (phospho T1141) antibody
    See all FHOD1 primary antibodies
  • Description
    Rabbit polyclonal to FHOD1 (phospho T1141)
  • Host species
    Rabbit
  • Specificity
    The immunogen sequence is highly conserved in rat and mouse FHOD1, and is well conserved in FHOD3 (Thr-1399).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues surrounding Thr 1141 of Human FHOD1.

  • Positive control
    • K562 cells and C2C12 cells stimulated with calyculin A.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab157589 was cross-adsorbed to unphosphorylated FHOD1 (Thr-1141) peptide before affinity purification using phospho-FHOD1 (Thr-1141) peptide (without carrier).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 127 kDa).

Target

  • Function
    Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.
  • Tissue specificity
    Ubiquitous. Highly expressed in spleen.
  • Sequence similarities
    Belongs to the formin homology family.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Domain
    Regulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • FH1/FH2 domain containing protein antibody
    • FH1/FH2 domain containing protein 1 antibody
    • FH1/FH2 domain-containing protein 1 antibody
    • Fhod1 antibody
    • FHOD1_HUMAN antibody
    • FHOS antibody
    • FHOS1 antibody
    • Formin homolog overexpressed in spleen 1 antibody
    • Formin homology 2 domain containing 1 antibody
    • Formin homology 2 domain containing protein 1 antibody
    • Formin homology 2 domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-FHOD1 (phospho T1141) antibody (ab157589) at 1/1000 dilution

    Lane 1 : K562 cells
    stimulated with calyculin A (100 nM) for 30 min
    Lane 2 : K562 cells stimulated with calyculin A (100 nM) for 30 min, then treated with lambda phosphatase

    Predicted band size: 127 kDa

References

ab157589 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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