Overview

  • Product nameAnti-FHOD3 antibody
  • Description
    Rabbit polyclonal to FHOD3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 390 - 439 (SGKFSGSSPA PPSQPQGLSY AEDAAEHENM KAVLKTSSPS VEDATPALGV) of Human FHOD3 (AAH81563).

  • Positive control
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActin-organizing protein that may cause stress fiber formation together with cell elongation.
  • Tissue specificityExpressed in the heart, kidney and brain.
  • Sequence similaritiesBelongs to the formin homology family.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Cellular localizationCytoplasm > cytoskeleton. Main part of the protein localizes to actin fibers and the remaining part displays filamentous staining.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22717 antibody
    • Formactin2 antibody
    • FH1/FH2 domain containing protein 3 antibody
    • FH1/FH2 domain-containing protein 3 antibody
    • Fhod3 antibody
    • FHOD3_HUMAN antibody
    • FHOS2 antibody
    • FLJ22297 antibody
    • Formactin-2 antibody
    • Formin homolog overexpressed in spleen 2 antibody
    • Formin homology 2 domain containing 3 antibody
    • hFHOS2 antibody
    • KIAA1695 antibody
    • mKIAA1695 antibody
    see all

Anti-FHOD3 antibody images

  • Anti-FHOD3 antibody (ab105651) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 60 kDa
    The band at 60kD may be either a non-specific protein or a degradation product of the larger ~155-160kD FHOD3 protein. There are no known cleavage products or isoforms known to be around this size. Based on the peptide immunogen used to raise this antibody, this band may be isoform 2 of human CARD14 (434aa; ~82% homology).

    Gel concetration: 12%
  • ab105651, at 8 µg/ml, staining FHOD3 in paraffin embeded Human kidney tissue by Immunohistochemistry. Cells with positive label are the epithelial cells from renal tubules (indicated by arrows). Magnification: 400X.

References for Anti-FHOD3 antibody (ab105651)

This product has been referenced in:
  • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue (Embryonic Heart Cryo-Section)
Permeabilization Yes - Tween-20
Specification Embryonic Heart Cryo-Section
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4%-12%)
Loading amount 10 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2016

Thank you for contacting us.

This band at ˜60kDa has been seen using this product and is on our website (http://www.abcam.com/FHOD3-antibody-ab105651.html#FHOD3-Primary-antibodies-ab105651-1.jpg).The band at 60kD may be either a non-specific...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"