Overview

  • Product nameAnti-FIBIN antibody
    See all FIBIN primary antibodies
  • Description
    Rabbit polyclonal to FIBIN
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: TLHHYFVPDG DYEENDDPEK CQLLFRVSDH RRCSQGEGSQ VGSLLSLTLR EEFTVLGRQV EDAGRVLEGI SKSISYD, corresponding to amino acids 32-108 of Human FIBIN.

  • Positive control
    • FIBIN over-expression HEK293T Lysate; Human placenta tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-FIBIN antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122621 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122621 at 1/50 dilution staining FIBIN in paraffin-embedded Human placenta tissue by Immunohistochemistry.
  • All lanes : Anti-FIBIN antibody (ab122621) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : FIBIN Over-expression HEK293T Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Developed using the ECL technique

References for Anti-FIBIN antibody (ab122621)

ab122621 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122621.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"