Overview

  • Product nameAnti-Fibrillarin antibody [28F2]
    See all Fibrillarin primary antibodies
  • Description
    Mouse monoclonal [28F2] to Fibrillarin
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Nuclear preparation (S. cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.

  • General notesPfam number: PF01269

Properties

Applications

Our Abpromise guarantee covers the use of ab4575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IP
  • Application notesIF: 1/2000 - 1/20000 (yeast cells).
    IP: Use 2-4 µl for an amount of lysate derived from 5 OD600 units of yeast culture.
    WB: 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
    • Sequence similaritiesBelongs to the methyltransferase superfamily. Fibrillarin family.
    • Post-translational
      modifications
      By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
    • Cellular localizationNucleus, nucleolus. Fibrillar region of the nucleolus.
    • Information by UniProt
    • Alternative names
      • 34 kD nucleolar scleroderma antigen antibody
      • 34 kDa nucleolar scleroderma antigen antibody
      • Fbl antibody
      • FBRL_HUMAN antibody
      • FIB antibody
      • FIB1 antibody
      • FLRN antibody
      • Histone-glutamine methyltransferase antibody
      • Nop1p antibody
      • RNA U3 small nucleolar interacting protein 1 antibody
      • RNU3IP1 antibody
      • rRNA 2' O methyltransferase fibrillarin antibody
      • rRNA 2'-O-methyltransferase fibrillarin antibody
      see all

    References for Anti-Fibrillarin antibody [28F2] (ab4575)

    This product has been referenced in:
    • Herzig B  et al. Bällchen is required for self-renewal of germline stem cells in Drosophila melanogaster. Biol Open 3:510-21 (2014). Read more (PubMed: 24876388) »
    • Merl J  et al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res 38:3068-80 (2010). ICC/IF . Read more (PubMed: 20100801) »

    See all 2 Publications for this product

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"